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miRNA Detection by Stem-Loop RT-qPCR in Studying microRNA Biogenesis and microRNA Responsiveness to Abiotic Stresses.

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第一作者： Aleksandra,Smoczynska

第一单位： Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poznań, Poland.

作者： Aleksandra,Smoczynska ^[1] ; Pawel,Sega ^[1] ; Agata,Stepien ^[1] ; Katarzyna,Knop ^[1] ; Artur,Jarmolowski ^[1] ; Andrzej,Pacak ^[2] ; Zofia,Szweykowska-Kulinska ^[3]

作者单位： Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poznań, Poland. ^[1] Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poznań, Poland. apacak@amu.edu.pl. ^[2] Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poznań, Poland. zofszwey@amu.edu.pl. ^[3]

关键词 Probes RT-ddPCR RT-qPCR Stem-loop primer TaqMan microRNA

主题词微RNAs(MicroRNAs);植物(Plants);RNA, 植物(RNA, Plant);逆转录聚合酶链反应(Reverse Transcriptase Polymerase Chain Reaction);逆转录作用(Reverse Transcription);应激, 生理学(Stress, Physiological)

DOI 10.1007/978-1-4939-9042-9_10

PMID 30701497

发布时间 2019-06-27

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