A high-throughput protocol for isolating cell-free circulating tumor DNA from peripheral blood.

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作者： Pawan K,Pandoh ^[1] ; Richard D,Corbett ^[1] ; Helen,McDonald ^[1] ; Miguel,Alcaide ^[2] ; Heather,Kirk ^[1] ; Eva,Trinh ^[1] ; Simon,Haile ^[1] ; Tina,MacLeod ^[1] ; Duane,Smailus ^[1] ; Steve,Bilobram ^[1] ; Andrew J,Mungall ^[1] ; Yussanne,Ma ^[1] ; Richard A,Moore ^[1] ; Robin,Coope ^[1] ; Yongjun,Zhao ^[1] ; Steven Jm,Jones ^[1] ; Robert A,Holt ^[3] ; Aly,Karsan ^[2] ; Ryan D,Morin ^[1] ; Marco A,Marra ^[3]

作者单位： Canada's Michael Smith Genome Sciences Centre, BC Cancer, 675 West 10th Avenue, Vancouver, BC, Canada. ^[1] Department of Molecular Biology & Biochemistry, Simon Fraser University, Burnaby, BC, Canada. ^[2] Department of Medical Genetics, Faculty of Medicine, University of British Columbia, Vancouver, Canada. ^[3] Department of Pathology & Laboratory Medicine, University of British Columbia, Vancouver, Canada. ^[4]

关键词 cfDNA ctDNA library construction liquid biopsy next-generation sequencing target capture

主题词高通量核苷酸序列分析(High-Throughput Nucleotide Sequencing);高通量筛选分析(High-Throughput Screening Assays);人类(Humans);突变(Mutation);肿瘤(Neoplasms)

DOI 10.2144/btn-2018-0148

PMID 30744412

发布时间 2020-01-14

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准确一般字义偏差语意偏离译文作用小提交