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Simple and efficient genome recombineering using kil counter-selection in Escherichia coli.

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第一作者： Wei,Chen

第一单位： Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China; Beijing Institute of Biotechnology, Beijing 100850, China.

作者： Wei,Chen ^[1] ; Yujuan,Li ^[2] ; Guangjin,Wu ^[2] ; Lingfeng,Zhao ^[2] ; Li,Lu ^[2] ; Peng,Wang ^[3] ; Jianguang,Zhou ^[3] ; Cheng,Cao ^[4] ; Shanhu,Li ^[5]

作者单位： Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China; Beijing Institute of Biotechnology, Beijing 100850, China. ^[1] Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China. ^[2] Beijing Institute of Biotechnology, Beijing 100850, China. ^[3] Beijing Institute of Biotechnology, Beijing 100850, China. Electronic address: cao_c@sohu.com. ^[4] Beijing Institute of Biotechnology, Beijing 100850, China. Electronic address: lishanhu6@163.com. ^[5]

关键词 Counter-selection Metabolic engineering Recombineering

医学主题词大肠杆菌(Escherichia coli);大肠杆菌蛋白质类(Escherichia coli Proteins);基因组, 细菌(Genome, Bacterial);重组, 遗传(Recombination, Genetic)

DOI 10.1016/j.jbiotec.2019.01.024

PMID 30768999

发布时间 2019-07-24

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