Determination of the activity of uracil-DNA glycosylase by using two-tailed reverse transcription PCR and gold nanoparticle-mediated silver nanocluster fluorescence: a new method for gene therapy-related enzyme detection.

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第一作者： Kai,Zhang

第一单位： Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, Jiangsu, China. zhangkai@jsinm.org.

作者： Kai,Zhang ^[1] ; Wanting,Huang ^[2] ; Yue,Huang ^[3] ; Ke,Wang ^[2] ; Xue,Zhu ^[2] ; Minhao,Xie ^[4]

作者单位： Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, Jiangsu, China. zhangkai@jsinm.org. ^[1] Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, Jiangsu, China. ^[2] College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, China. ^[3] Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, Jiangsu, China. xieminhao@jsinm.org. ^[4]

关键词 Entropy-driven Fluorescence Silver nanoclusters T7 RNA polymerase Uracil-DNA glycosylase