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A general platform for efficient extracellular expression and purification of Fab from Escherichia coli.

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第一作者： Manyu,Luo

第一单位： Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.

作者： Manyu,Luo ^[1] ; Meiqi,Zhao ^[1] ; Cedric,Cagliero ^[2] ; Hua,Jiang ^[2] ; Yueqing,Xie ^[2] ; Jianwei,Zhu ^[3] ; Hui,Yang ^[1] ; Mengxiao,Zhang ^[1] ; Ying,Zheng ^[1] ; Yunsheng,Yuan ^[1] ; Zixiu,Du ^[4] ; Huili,Lu ^[5]

作者单位： Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. ^[1] Jecho Laboratories, Inc., 7320 Executive Way, Frederick, MD, 21704, USA. ^[2] Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.;Jecho Laboratories, Inc., 7320 Executive Way, Frederick, MD, 21704, USA. ^[3] School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. ^[4] Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. roadeer@sjtu.edu.cn. ^[5]

关键词 E. coli Extracellular production Fab STII phoA