Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas.

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作者： Adam E,Dolan ^[1] ; Zhonggang,Hou ^[2] ; Yibei,Xiao ^[3] ; Max J,Gramelspacher ^[2] ; Jaewon,Heo ^[2] ; Sara E,Howden ^[4] ; Peter L,Freddolino ^[5] ; Ailong,Ke ^[6] ; Yan,Zhang ^[7]

作者单位： Department of Molecular Biology and Genetics, Cornell University, 253 Biotechnology Building, Ithaca, NY 14853, USA. ^[1] Department of Biological Chemistry, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI 48109, USA. ^[2] Department of Molecular Biology and Genetics, Cornell University, 253 Biotechnology Building, Ithaca, NY 14853, USA; State Key Laboratory of Natural Medicines, Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China. ^[3] Murdoch Children's Research Institute, Flemington Rd., Parkville, VIC 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC 3052, Australia. ^[4] Department of Biological Chemistry, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI 48109, USA; Department of Computational Medicine and Bioinformatics, University of Michigan, 100 Washtenaw Avenue, Ann Arbor, MI 48109, USA. ^[5] Department of Molecular Biology and Genetics, Cornell University, 253 Biotechnology Building, Ithaca, NY 14853, USA. Electronic address: ailong.ke@cornell.edu. ^[6] Department of Biological Chemistry, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI 48109, USA. Electronic address: yzhangbc@med.umich.edu. ^[7]

关键词 CRISPR-Cas Cas3 Cascade RNA-guided chromosome embryonic stem cell genome editing large genome deletion long-range type I CRISPR

主题词内切核酸酶类(Endonucleases);大肠杆菌(Escherichia coli);基因组, 人(Genome, Human);基因组学(Genomics);人类(Humans);核糖核蛋白类(Ribonucleoproteins);序列缺失(Sequence Deletion)

DOI 10.1016/j.molcel.2019.03.014

PMID 30975459

发布时间 2024-01-04

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