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Bioconjugation as a smart immobilization approach for α-amylase enzyme using stimuli-responsive Eudragit-L100 polymer: a robust biocatalyst for applications in pharmaceutical industry.

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第一作者： Heidi Mohamed,Abdel-Mageed

第一单位： a Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre , Cairo , Egypt.;b Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt (FUE) , Cairo , Egypt.

作者： Heidi Mohamed,Abdel-Mageed ^[1] ; Rasha Ali,Radwan ^[2] ; Nermeen Zakaria,AbuelEzz ^[3] ; Hebatallah Ahmed,Nasser ^[4] ; Aliaa Ali,El Shamy ^[4] ; Rana M,Abdelnaby ^[5] ; Nesrine Abdelrehim,El Gohary ^[6]

作者单位： a Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre , Cairo , Egypt.;b Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt (FUE) , Cairo , Egypt. ^[1] c Biochemistry and Biotechnology Department, Faculty of Pharmacy and Drug Technology, Heliopolis University , Cairo , Egypt. ^[2] d Biochemistry Department, College of Pharmaceutical Sciences & Drug Manufacturing, Misr University for Science and Technology , Cairo , Egypt. ^[3] e Microbiology and Public Health Department, Faculty of Pharmacy and Drug Technology, Heliopolis University , Egypt. ^[4] f Pharmaceutical Chemistry Department, Faculty of Pharmacy and Drug Technology, Heliopolis University , Egypt. ^[5] g Pharmaceutical Chemistry Department, Faculty of Pharmacy and Biotechnology, German University in Cairo , Cairo , Egypt. ^[6]

关键词 Biocatalyst Eudragit stimuli-responsive polymer Immobilized enzymes stability carbodiimide bio-conjugation covalent immobilization starch processing α-amylase enzyme