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Manipulation of charge distribution in the arginine and glutamate clusters of the OmpG pore alters sugar specificity and ion selectivity.

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第一作者： Christine,Schmitt

第一单位： Division of Biochemistry and Applied Protein Center Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany; Department Biology, Division of Plant Physiology, Philipps-University Marburg, D-35043 Marburg, Germany. Electronic address: christine.schmitt@fau.de.

作者： Christine,Schmitt ^[1] ; Jayesh Arun,Bafna ^[2] ; Benedikt,Schmid ^[3] ; Stefan,Klingl ^[4] ; Steffen,Baier ^[5] ; Birgit,Hemmis ^[6] ; Richard,Wagner ^[7] ; Mathias,Winterhalter ^[8] ; Lars M,Voll ^[9]

作者单位： Division of Biochemistry and Applied Protein Center Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany; Department Biology, Division of Plant Physiology, Philipps-University Marburg, D-35043 Marburg, Germany. Electronic address: christine.schmitt@fau.de. ^[1] Department of Life Sciences and Chemistry, Jacobs University Bremen, D-28719 Bremen, Germany. Electronic address: j.bafna@jacobs-university.de. ^[2] Division of Biotechnology and Applied Protein Center Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany. Electronic address: benedikt.schmid@fau.de. ^[3] Division of Biotechnology and Applied Protein Center Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany. Electronic address: sklingl@biologie.uni-erlangen.de. ^[4] Division of Biochemistry and Applied Protein Center Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany. ^[5] Department of Biology and Chemistry, University of Osnabrück, D-49069 Osnabrück, Germany. ^[6] Department of Life Sciences and Chemistry, Jacobs University Bremen, D-28719 Bremen, Germany; Department of Biology and Chemistry, University of Osnabrück, D-49069 Osnabrück, Germany. Electronic address: ri.wagner@jacobs-university.de. ^[7] Department of Life Sciences and Chemistry, Jacobs University Bremen, D-28719 Bremen, Germany. Electronic address: m.winterhalter@jacobs-university.de. ^[8] Division of Biochemistry and Applied Protein Center Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany; Department Biology, Division of Plant Physiology, Philipps-University Marburg, D-35043 Marburg, Germany. Electronic address: lars.voll@biologie.uni-marburg.de. ^[9]

关键词 E. coli Ion selectivity Liposome swelling assay Porin engineering Reversal potential Sugar specificity