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首页> Enzyme and microbial technology> Semi-rationally engineered variants of S-adenosylmethionine synthetase from Escherichia coli with reduced product inhibition and improved catalytic activity.

Semi-rationally engineered variants of S-adenosylmethionine synthetase from Escherichia coli with reduced product inhibition and improved catalytic activity.

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作者： Xiu,Wang ^[1] ; Yiqi,Jiang ^[1] ; Mianbin,Wu ^[1] ; Li,Zhu ^[1] ; Lirong,Yang ^[1] ; Jianping,Lin ^[2]

作者单位： Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China. ^[1] Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China. Electronic address: linjp@zju.edu.cn. ^[2]

关键词 Enzyme activity Product inhibition S-adenosylmethionine synthetase Saturated mutagenesis Semi-rational modification

主题词催化作用(Catalysis);催化域(Catalytic Domain);大肠杆菌(Escherichia coli);大肠杆菌蛋白质类(Escherichia coli Proteins);动力学(Kinetics);甲硫氨酸(Methionine);甲硫氨酸腺苷转移酶(Methionine Adenosyltransferase);模型, 分子(Models, Molecular);S-腺苷甲硫氨酸(S-Adenosylmethionine)

DOI 10.1016/j.enzmictec.2019.05.012

PMID 31307578

发布时间 2019-11-27

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Enzyme and microbial technology

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