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Standardization of the preanalytical phase of DNA extraction from fixed tissue for next-generation sequencing analyses.

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第一作者： Olga,Kofanova

第一单位： IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: olga.kofanova@ibbl.lu.

作者： Olga,Kofanova ^[1] ; Camille,Bellora ^[2] ; Sonia,Garcia Frasquilho ^[3] ; Laurent,Antunes ^[4] ; Gael,Hamot ^[5] ; Conny,Mathay ^[6] ; Kathleen,Mommaerts ^[7] ; Arnaud,Muller ^[8] ; Brian,DeWitt ^[9] ; Fay,Betsou ^[10]

作者单位： IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: olga.kofanova@ibbl.lu. ^[1] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: camille.bellora@ibbl.lu. ^[2] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: sonia.frasquilho@ibbl.lu. ^[3] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: laurent.antunes@lns.etat.lu. ^[4] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: gael.hamot@ibbl.lu. ^[5] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: conny.mathay@asta.etat.lu. ^[6] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: kathleen.mommaerts@ibbl.lu. ^[7] LIH (Luxembourg Institute for Health), 1 A-B Rue Thomas Edison, 1445 Strassen, Luxembourg. Electronic address: arnaud.muller@lih.lu. ^[8] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: brian.dewitt@ibbl.lu. ^[9] IBBL (Integrated BioBank of Luxembourg), 1 rue Louis Rech, L-3555 Dudelange, Luxembourg. Electronic address: fay.betsou@ibbl.lu. ^[10]

关键词 DNA extraction FFPE Next-Generation sequencing Preanalytics Quality control Tissue

医学主题词 DNA(DNA);高通量核苷酸序列分析(High-Throughput Nucleotide Sequencing);人类(Humans);质量控制(Quality Control);组织固定(Tissue Fixation)

DOI 10.1016/j.nbt.2019.07.005

PMID 31398512

发布时间 2020-02-24

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准确一般字义偏差语意偏离译文作用小提交