Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in <i>Leishmania</i>.

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作者： Wen-Wei,Zhang ^[1] ; Greg,Matlashewski ^[2]

作者单位： Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada. ^[1] Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada greg.matlashewski@mcgill.ca. ^[2]

关键词 CRISPR-Cas9 DNA polymerase theta Leishmania SaCas9 double-strand DNA break repair gene deletion gene targeting microhomology-mediated end joining miltefosine transporter gene nonhomologous end joining parasite single-strand annealing

主题词 DNA断裂, 双链(DNA Breaks, Double-Stranded);DNA修复(DNA Repair);DNA, 原虫(DNA, Protozoan);DNA, 单链(DNA, Single-Stranded);基因组, 原虫(Genome, Protozoan);利什曼原虫属(Leishmania);重组, 遗传(Recombination, Genetic);金黄色葡萄球菌(Staphylococcus aureus)

DOI 10.1128/mSphere.00408-19

PMID 31434745

发布时间 2020-03-09

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Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in <i>Leishmania</i>.

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Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in <i>Leishmania</i>.

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