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Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells.

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第一作者: Xi,Wang
第一单位: Ministry of Education Key Laboratory of Diagnostic Medicine, and School of Laboratory and Diagnostic Medicine , Chongqing Medical University , Chongqing , 400016 , China.;Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.
作者: Xi,Wang [1] ; Chengfu,Yuan [2] ; Bo,Huang [3] ; Jiaming,Fan [1] ; Yixiao,Feng [4] ; Alexander J,Li [5] ; Bo,Zhang [6] ; Yan,Lei [4] ; Zhenyu,Ye [7] ; Ling,Zhao [4] ; Daigui,Cao [8] ; Lijuan,Yang [6] ; Di,Wu [5] ; Xian,Chen [9] ; Bin,Liu [10] ; William,Wagstaff [5] ; Fang,He [4] ; Xiaoxing,Wu [4] ; Huaxiu,Luo [11] ; Jing,Zhang [4] ; Meng,Zhang [12] ; Rex C,Haydon [5] ; Hue H,Luu [5] ; Michael J,Lee [5] ; Jennifer,Moriatis Wolf [5] ; Ailong,Huang [13] ; Tong-Chuan,He [5] ; Zongyue,Zeng [14]
作者单位: Ministry of Education Key Laboratory of Diagnostic Medicine, and School of Laboratory and Diagnostic Medicine , Chongqing Medical University , Chongqing , 400016 , China.;Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States. [1] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Department of Biochemistry and Molecular Biology , China Three Gorges University School of Medicine , Yichang , 443002 , China. [2] Ministry of Education Key Laboratory of Diagnostic Medicine, and School of Laboratory and Diagnostic Medicine , Chongqing Medical University , Chongqing , 400016 , China.;Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Department of Clinical Laboratory Medicine , the Second Affiliated Hospital of Nanchang University , Nanchang , 330006 , China. [3] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;The Affiliated Hospitals of Chongqing Medical University , Chongqing , 400016 , China. [4] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States. [5] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Key Laboratory of Orthopaedic Surgery of Gansu Province, and the Departments of Orthopaedic Surgery and Obstetrics and Gynecology , the First and Second Hospitals of Lanzhou University , Lanzhou , 730030 , China. [6] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Department of General Surgery , the Second Affiliated Hospital of Soochow University , Suzhou , 215004 , China. [7] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;The Affiliated Hospitals of Chongqing Medical University , Chongqing , 400016 , China.;Department of Orthopaedic Surgery , Chongqing General Hospital , Chongqing , 400013 , China. [8] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Department of Clinical Laboratory Medicine , The Affiliated Hospital of Qingdao University , Qingdao , 266061 , China. [9] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;School of Life Sciences , Southwest University , Chongqing , 400715 , China. [10] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Department of Burn and Plastic Surgery , West China Hospital of Sichuan University , Chengdu , 610041 , China. [11] Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Department of Orthopaedic Surgery, The First Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , 510405 , China. [12] Key Laboratory of Molecular Biology for Infectious Diseases of The Ministry of Education of China, Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital , Chongqing Medical University , Chongqing , China. [13] Ministry of Education Key Laboratory of Diagnostic Medicine, and School of Laboratory and Diagnostic Medicine , Chongqing Medical University , Chongqing , 400016 , China.;Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine , The University of Chicago Medical Center , Chicago , Illinois 60637 , United States.;Key Laboratory of Molecular Biology for Infectious Diseases of The Ministry of Education of China, Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital , Chongqing Medical University , Chongqing , China. [14]
DOI 10.1021/acssynbio.9b00203
PMID 31465214
发布时间 2020-08-30
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ACS synthetic biology

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