Engineering protein production by rationally choosing a carbon and nitrogen source using E. coli BL21 acetate metabolism knockout strains.

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第一作者： Gema,Lozano Terol

第一单位： Department of Biochemistry and Molecular Biology (B) and Immunology, Faculty of Chemistry, University of Murcia, Campus of Espinardo, Regional Campus of International Excellence ''Campus Mare Nostrum'', P.O. Box 4021, 30100, Murcia, Spain.

作者： Gema,Lozano Terol ^[1] ; Julia,Gallego-Jara ^[2] ; Rosa Alba,Sola Martínez ^[1] ; Manuel,Cánovas Díaz ^[1] ; Teresa,de Diego Puente ^[3]

作者单位： Department of Biochemistry and Molecular Biology (B) and Immunology, Faculty of Chemistry, University of Murcia, Campus of Espinardo, Regional Campus of International Excellence ''Campus Mare Nostrum'', P.O. Box 4021, 30100, Murcia, Spain. ^[1] Department of Biochemistry and Molecular Biology (B) and Immunology, Faculty of Chemistry, University of Murcia, Campus of Espinardo, Regional Campus of International Excellence ''Campus Mare Nostrum'', P.O. Box 4021, 30100, Murcia, Spain. julia.gallego@um.es. ^[2] Department of Biochemistry and Molecular Biology (B) and Immunology, Faculty of Chemistry, University of Murcia, Campus of Espinardo, Regional Campus of International Excellence ''Campus Mare Nostrum'', P.O. Box 4021, 30100, Murcia, Spain. tdp@um.es. ^[3]

关键词 Acetate overflow Escherichia coli GFP Lysine acetylation Medium composition Recombinant proteins