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首页> Methods in molecular biology (Clifton, N.J.)> Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis.

Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis.

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第一作者： Jennifer,Eng

第一单位： Department of Biomedical Engineering and OHSU Center for Spatial Systems Biomedicine, Oregon Health and Science University, Portland, OR, USA.

作者： Jennifer,Eng ^[1] ; Guillaume,Thibault ^[1] ; Shiuh-Wen,Luoh ^[2] ; Joe W,Gray ^[3] ; Young Hwan,Chang ^[1] ; Koei,Chin ^[2]

作者单位： Department of Biomedical Engineering and OHSU Center for Spatial Systems Biomedicine, Oregon Health and Science University, Portland, OR, USA. ^[1] Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA. ^[2] Veterans Administration Portland Health Care System, Portland, OR, USA. ^[3] Department of Biomedical Engineering and OHSU Center for Spatial Systems Biomedicine, Oregon Health and Science University, Portland, OR, USA. ChinKoei@ohsu.edu. ^[4] Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA. ChinKoei@ohsu.edu. ^[5]

关键词 Breast cancer Cyclic IF Cyclic immunofluorescence Cyclic multiplexed-immunofluorescence (cmIF)Immunotherapy Tumor infiltrating lymphocytes (TILs)

主题词女(雌)性(Female);荧光抗体技术(Fluorescent Antibody Technique);人类(Humans);图像处理, 计算机辅助(Image Processing, Computer-Assisted);免疫疗法(Immunotherapy);淋巴细胞, 肿瘤浸润(Lymphocytes, Tumor-Infiltrating);石蜡包埋(Paraffin Embedding);单细胞分析(Single-Cell Analysis);组织固定(Tissue Fixation)

DOI 10.1007/978-1-4939-9773-2_24

PMID 31502168

发布时间 2021-02-11

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Methods in molecular biology (Clifton, N.J.)

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