Single-molecule analysis reveals rotational substeps and chemo-mechanical coupling scheme of <i>Enterococcus hirae</i> V<sub>1</sub>-ATPase.

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第一作者： Tatsuya,Iida

第一单位： Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan.;Department of Functional Molecular Science, School of Physical Sciences, SOKENDAI (Graduate University for Advanced Studies), Shonan Village, Hayama, Kanagawa 240-0193, Japan.

作者： Tatsuya,Iida ^[1] ; Yoshihiro,Minagawa ^[2] ; Hiroshi,Ueno ^[2] ; Fumihiro,Kawai ^[3] ; Takeshi,Murata ^[4] ; Ryota,Iino ^[5]

作者单位： Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan.;Department of Functional Molecular Science, School of Physical Sciences, SOKENDAI (Graduate University for Advanced Studies), Shonan Village, Hayama, Kanagawa 240-0193, Japan. ^[1] Department of Applied Chemistry, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. ^[2] Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan. ^[3] Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.;Japan Science and Technology Agency (JST), PRESTO, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan. ^[4] Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji-cho, Okazaki, Aichi 444-8787, Japan iino@ims.ac.jp.;Department of Functional Molecular Science, School of Physical Sciences, SOKENDAI (Graduate University for Advanced Studies), Shonan Village, Hayama, Kanagawa 240-0193, Japan. ^[5]

关键词 ATPase Enterococcus V1-ATPase biophysics ion pump molecular motor rotary motor rotational substep single-molecule biophysics vacuolar ATPase

主题词腺苷二磷酸(Adenosine Diphosphate);腺苷三磷酸(Adenosine Triphosphate);力学现象(Mechanical Phenomena);模型, 分子(Models, Molecular);蛋白质构象(Protein Conformation);旋转(Rotation);空泡质子转运ATP酶(Vacuolar Proton-Translocating ATPases)

DOI 10.1074/jbc.RA119.008947

PMID 31519751

发布时间 2021-02-05

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