High-Throughput Assessment of Kinome-wide Activation States.

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第一作者： Thierry,Schmidlin

第一单位： Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands; Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, the Netherlands.

作者： Thierry,Schmidlin ^[1] ; Donna O,Debets ^[1] ; Charlotte A G H,van Gelder ^[1] ; Kelly E,Stecker ^[1] ; Stamatia,Rontogianni ^[1] ; Bart L,van den Eshof ^[2] ; Kristel,Kemper ^[3] ; Esther H,Lips ^[4] ; Maartje,van den Biggelaar ^[2] ; Daniel S,Peeper ^[3] ; Albert J R,Heck ^[1] ; Maarten,Altelaar ^[5]

作者单位： Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands; Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, the Netherlands. ^[1] Sanquin Research, Department of Molecular and Cellular Hemostasis, Amsterdam, the Netherlands. ^[2] Division of Molecular Oncology, Oncode Institute, The Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands. ^[3] Department of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands. ^[4] Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands; Netherlands Proteomics Center, Padualaan 8, 3584 CH Utrecht, the Netherlands; Mass Spectrometry and Proteomics Facility, The Netherlands Cancer Institute, 1066 CX Amsterdam, the Netherlands. Electronic address: m.altelaar@uu.nl. ^[5]

关键词 SRM T-loop phosphorylation cancer kinase kinase activity phosphoproteomics proteomics signaling targeted mass spectrometry

主题词活组织检查, 针吸(Biopsy, Needle);乳腺肿瘤(Breast Neoplasms);酶激活(Enzyme Activation);女(雌)性(Female);高通量筛选分析(High-Throughput Screening Assays);人类(Humans);Jurkat细胞(Jurkat Cells);质谱分析法(Mass Spectrometry);肽类(Peptides);磷酰化(Phosphorylation);蛋白质组学(Proteomics);受体作用蛋白丝氨酸苏氨酸激酶类(Receptor-Interacting Protein Serine-Threonine Kinases);结果可重复性(Reproducibility of Results);敏感性与特异性(Sensitivity and Specificity);肿瘤坏死因子α(Tumor Necrosis Factor-alpha)

DOI 10.1016/j.cels.2019.08.005

PMID 31521607

发布时间 2021-01-10

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Cell systems

2019年9卷4期

366-374.e5页

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High-Throughput Assessment of Kinome-wide Activation States.

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High-Throughput Assessment of Kinome-wide Activation States.

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