Mass cytometry-based single-cell analysis of human stem cell reprogramming uncovers differential regulation of specific pluripotency markers.

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第一作者： Ilkyun,Im

第一单位： Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahag-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

作者： Ilkyun,Im ^[1] ; Ye Seul,Son ^[2] ; Kwang Bo,Jung ^[2] ; Insoo,Kang ^[3] ; Boon-Eng,Teh ^[4] ; Kyung-Bok,Lee ^[5] ; Mi-Young,Son ^[6] ; Janghwan,Kim ^[7]

作者单位： Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahag-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. ^[1] Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahag-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Functional Genomics, Korea Research Institute of Bioscience and Biotechnology School of Bioscience, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. ^[2] Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520; Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520; Department of Orthopedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut 06520. ^[3] Fluidigm Corporation, South San Francisco, California 94080-7603. ^[4] Center for Research Equipment, Korea Basic Science Institute, 162 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju 28119, Republic of Korea. ^[5] Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahag-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Functional Genomics, Korea Research Institute of Bioscience and Biotechnology School of Bioscience, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address: myson@kribb.re.kr. ^[6] Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahag-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Department of Functional Genomics, Korea Research Institute of Bioscience and Biotechnology School of Bioscience, Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address: janghwan.kim@kribb.re.kr. ^[7]

关键词 OCT4 TRA-1–60 cell cycle computational biology iPS cell iPSC induced pluripotent stem cell mass cytometry pluripotency reprogramming single-cell analysis spanning-tree progression analysis of density-normalized events (SPADE)