Characterization of the internal translation initiation region in monoclonal antibodies expressed in <i>Escherichia coli</i>.

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作者： Erik M,Leith ^[1] ; William B,O'Dell ^[2] ; Na,Ke ^[3] ; Colleen,McClung ^[3] ; Mehmet,Berkmen ^[3] ; Christina,Bergonzo ^[4] ; Robert G,Brinson ^[4] ; Zvi,Kelman ^[5]

作者单位： Biomolecular Labeling Laboratory, National Institute of Standards and Technology and Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850. ^[1] Biomolecular Labeling Laboratory, National Institute of Standards and Technology and Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850; National Institute of Standards and Technology, Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850. ^[2] New England Biolabs, Ipswich, Massachusetts 01938. ^[3] National Institute of Standards and Technology, Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850. ^[4] Biomolecular Labeling Laboratory, National Institute of Standards and Technology and Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850; National Institute of Standards and Technology, Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850. Electronic address: zkelman@umd.edu. ^[5]

关键词 Escherichia coli (E. coli)GTG codon NISTmAb Shine-Dalgarno sequence codon optimization mAb monoclonal antibody protein expression recombinant protein expression translation initiation translation regulation

主题词大肠杆菌(Escherichia coli);免疫球蛋白重链(Immunoglobulin Heavy Chains);肽链起始, 翻译(Peptide Chain Initiation, Translational);重组蛋白质类(Recombinant Proteins)

DOI 10.1074/jbc.RA119.011008

PMID 31604819

发布时间 2021-03-14

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准确一般字义偏差语意偏离译文作用小提交