Application of CRISPR/Cas9 editing and digital droplet PCR in human iPSCs to generate novel knock-in reporter lines to visualize dopaminergic neurons.

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第一作者： Christa,Überbacher

第一单位： Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy; Department of Biomedical, Metabolic and Neural Sciences, Università di Modena e Reggio Emilia, Modena, Italy. Electronic address: christa.ueberbacher@i-med.ac.at.

作者： Christa,Überbacher ^[1] ; Julia,Obergasteiger ^[2] ; Mattia,Volta ^[2] ; Serena,Venezia ^[2] ; Stefan,Müller ^[3] ; Isabella,Pesce ^[4] ; Sara,Pizzi ^[2] ; Giulia,Lamonaca ^[2] ; Anne,Picard ^[2] ; Giada,Cattelan ^[2] ; Giorgio,Malpeli ^[5] ; Michele,Zoli ^[6] ; Dayne,Beccano-Kelly ^[7] ; Rowan,Flynn ^[8] ; Richard,Wade-Martins ^[7] ; Peter P,Pramstaller ^[2] ; Andrew A,Hicks ^[2] ; Sally A,Cowley ^[9] ; Corrado,Corti ^[10]

作者单位： Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy; Department of Biomedical, Metabolic and Neural Sciences, Università di Modena e Reggio Emilia, Modena, Italy. Electronic address: christa.ueberbacher@i-med.ac.at. ^[1] Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy. ^[2] Institute of Human Genetics, Munich University Hospital, Ludwig-Maximilians University, Munich, Germany. ^[3] CIBIO - Centre for Integrative Biology, Università degli Studi di Trento, Trento, Italy. ^[4] Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, Section of Surgery, University of Verona, Verona, Italy; Department of Diagnostics and Public Health, University of Verona, Verona, Italy. ^[5] Department of Biomedical, Metabolic and Neural Sciences, Università di Modena e Reggio Emilia, Modena, Italy; Center for Neuroscience and Neurotechnology, University of Modena and Reggio Emilia, Modena, Italy. ^[6] Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK; Oxford Parkinson's Disease Centre, University of Oxford, Oxford, UK. ^[7] James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK. ^[8] Oxford Parkinson's Disease Centre, University of Oxford, Oxford, UK; James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK. ^[9] Institute for Biomedicine, Eurac Research, Affiliated Institute of the University of Lübeck, Bolzano, Italy. Electronic address: corrado.corti@eurac.edu. ^[10]

关键词 CRISPR/Cas9 Dopaminergic neurons Human induced pluripotent stem cells Knock-in, Digital droplet PCR, Fluorescent reporter, FACS