The pentose phosphate pathway of cellulolytic clostridia relies on 6-phosphofructokinase instead of transaldolase.

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作者： Jeroen G,Koendjbiharie ^[1] ; Shuen,Hon ^[2] ; Martin,Pabst ^[3] ; Robert,Hooftman ^[4] ; David M,Stevenson ^[5] ; Jingxuan,Cui ^[6] ; Daniel,Amador-Noguez ^[7] ; Lee R,Lynd ^[8] ; Daniel G,Olson ^[2] ; Richard,van Kranenburg ^[9]

作者单位： Corbion, 4206 AC Gorinchem, The Netherlands. ^[1] Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755; Center for Bioenergy Innovation, Oak Ridge National Laboratories, Oak Ridge, Tennessee 37830. ^[2] Cell Systems Engineering, Delft University of Technology, 2629 HZ Delft, The Netherlands. ^[3] Laboratory of Microbiology, Wageningen University & Research, 6708 WE Wageningen, The Netherlands. ^[4] Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706. ^[5] Center for Bioenergy Innovation, Oak Ridge National Laboratories, Oak Ridge, Tennessee 37830; Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, 03755. ^[6] Center for Bioenergy Innovation, Oak Ridge National Laboratories, Oak Ridge, Tennessee 37830; Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706. ^[7] Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire 03755; Center for Bioenergy Innovation, Oak Ridge National Laboratories, Oak Ridge, Tennessee 37830; Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, 03755. ^[8] Corbion, 4206 AC Gorinchem, The Netherlands; Laboratory of Microbiology, Wageningen University & Research, 6708 WE Wageningen, The Netherlands. Electronic address: richard.van.kranenburg@corbion.com. ^[9]

关键词 Clostridium thermocellum Clostridium thermosuccinogenes bacterial metabolism enzyme kinetics mass spectrometry (MS)pentose phosphate pathway (PPP)phosphofructokinase pyrophosphate sedoheptulose 1,7-bisphosphate transaldolase