Processing and integration of functionally oriented prespacers in the <i>Escherichia coli</i> CRISPR system depends on bacterial host exonucleases.

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作者： Anita,Ramachandran ^[1] ; Lesley,Summerville ^[1] ; Brian A,Learn ^[1] ; Lily,DeBell ^[1] ; Scott,Bailey ^[2]

作者单位： Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205. ^[1] Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205; Department of Biophysics and Biophysical Chemistry, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205. Electronic address: scott.bailey@jhu.edu. ^[2]

关键词 3'-5' exonuclease CRISPR-RNA (crRNA)CRISPR/Cas DNA DnaQ bacterial immunity deoxyribonuclease (DNase)integrase prespacer processing primed adaptation protospacer adjacent motif (PAM)

主题词碱基序列(Base Sequence);DNA聚合酶Ⅲ(DNA Polymerase III);大肠杆菌(Escherichia coli);大肠杆菌蛋白质类(Escherichia coli Proteins);外切核酸酶类(Exonucleases);序列分析, DNA(Sequence Analysis, DNA)

DOI 10.1074/jbc.RA119.012196

PMID 31914418

发布时间 2021-03-29

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