A streamlined workflow for twoplexing of N-linked glycan analysis using light (12C6) and heavy (13C6) isotopologues of 3-aminobenzenesulfonic acid.

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作者： Zoltan,Szabo ^[1] ; Shaheer H,Khan ^[1] ; Philip J,Widdowson ^[2] ; Dietmar,Reusch ^[3] ; Rosa,Viner ^[4] ; Sara,Carillo ^[5] ; Andreas,Huhmer ^[4] ; Jonathan,Bones ^[6]

作者单位： Thermo Fisher Scientific, 180 Oyster Point Blvd, South San Francisco, CA, 94080, United States. ^[1] Thermo Fisher Scientific, Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, United Kingdom. ^[2] Roche Diagnostics GmbH, 2 Nonnenwald, Penzberg, 82377, Germany. ^[3] Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, CA, 95134, United States. ^[4] National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co. Dublin, A94 X099, Ireland. ^[5] National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co. Dublin, A94 X099, Ireland; School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin 4, D04 V1W8, Ireland. Electronic address: jonathan.bones@nibrt.ie. ^[6]

关键词 Fluorescence detection Glycan analysis Glycan mass spectrometry Isotopic labelling of oligosaccharides Liquid chromatography mass spectrometry Quantitative glycan analysis Stable isotope labelling

主题词碳同位素(Carbon Isotopes);质谱分析法(Mass Spectrometry);多糖类(Polysaccharides);对氨基苯磺酸类(Sulfanilic Acids)

DOI 10.1016/j.aca.2019.11.055

PMID 31986272

发布时间 2020-11-12

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