Bacterial expression, purification, and initial characterization of a full-length Cas13b protein from Porphyromonas gingivalis.

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作者： Jiang,Zhu ^[1] ; Xia,Zhou ^[1] ; Xiaolan,Huang ^[2] ; Zhihua,Du ^[3]

作者单位： Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL, 62901, USA. ^[1] School of of Computing, Southern Illinois University, Carbondale, IL, 62901, USA. ^[2] Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL, 62901, USA. Electronic address: zdu@chem.siu.edu. ^[3]

关键词 CRISPR Cas13b HEPN Pre-crRNA processing mRNA knockdown

主题词内切核酸酶类(Endonucleases);大肠杆菌(Escherichia coli);龈紫单胞菌(Porphyromonas gingivalis);重组蛋白质类(Recombinant Proteins)

DOI 10.1016/j.pep.2020.105588

PMID 32006655

发布时间 2020-09-29

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