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首页> Journal of biotechnology> IGF1 inclusion bodies: A QbD based process approach for efficient USP as well as early DSP unit operations.

IGF1 inclusion bodies: A QbD based process approach for efficient USP as well as early DSP unit operations.

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作者： Karl F J,Metzger ^[1] ; Wolfgang,Padutsch ^[2] ; Alexander,Pekarsky ^[3] ; Julian,Kopp ^[3] ; Alexei M,Voloshin ^[4] ; Harald,Kühnel ^[2] ; Michael,Maurer ^[5]

作者单位： University of Applied Sciences Campus Vienna, Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Bioprocess Engineering, Vienna, Austria; University of Natural Resources and Life Sciences Vienna, Department of Biotechnology, Vienna, Austria. ^[1] University of Applied Sciences Campus Vienna, Life Sciences, Vienna, Austria. ^[2] Vienna University of Technology, Institute of Chemical, Environmental and Bioscience Engineering, Vienna, Austria. ^[3] 3M Company, Separation and Purification Sciences Division, Saint Paul, USA. ^[4] University of Applied Sciences Campus Vienna, Life Sciences, Vienna, Austria; Austrian Centre of Industrial Biotechnology, Bioprocess Engineering, Vienna, Austria. Electronic address: michael.maurer@fh-campuswien.ac.at. ^[5]

关键词 Adsorptive filter Early DSP Escherichia coli Inclusion body QbD Refolding

主题词吸附(Adsorption);生物反应器(Bioreactors);化学现象(Chemical Phenomena);色谱法(Chromatography);内毒素类(Endotoxins);大肠杆菌(Escherichia coli);过滤(Filtration);基因表达调控, 细菌(Gene Expression Regulation, Bacterial);氢离子浓度(Hydrogen-Ion Concentration);包涵体(Inclusion Bodies);胰岛素样生长因子Ⅰ(Insulin-Like Growth Factor I);粒子大小(Particle Size);蛋白质折叠(Protein Folding);重组蛋白质类(Recombinant Proteins);溶解度(Solubility);温度(Temperature);时间因素(Time Factors)

DOI 10.1016/j.jbiotec.2020.02.014

PMID 32114153

发布时间 2020-10-19

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Journal of biotechnology

23-34页

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