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Highly Parallel Profiling of Cas9 Variant Specificity.

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第一作者： Jonathan L,Schmid-Burgk

第一单位： Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

作者： Jonathan L,Schmid-Burgk ^[1] ; Linyi,Gao ^[1] ; David,Li ^[1] ; Zachary,Gardner ^[1] ; Jonathan,Strecker ^[1] ; Blake,Lash ^[1] ; Feng,Zhang ^[2]

作者单位： Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. ^[1] Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA. Electronic address: zhang@broadinstitute.org. ^[2]

关键词 CRISPR-Cas9 Cas9 specificity variants double-strand break detection off-target analysis tagmentation

医学主题词 DNA断裂, 双链(DNA Breaks, Double-Stranded);遗传变异(Genetic Variation);HEK293细胞(HEK293 Cells);人类(Humans);K562细胞(K562 Cells)

DOI 10.1016/j.molcel.2020.02.023

PMID 32187529

发布时间 2024-01-04

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