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High-Throughput Sequencing (HTS) of newly synthetized RNAs enables one shot detection and identification of live mycoplasmas and differentiation from inert nucleic acids.

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第一作者： Céline,Desbrousses

第一单位： PathoQuest, Paris, France.

作者： Céline,Desbrousses ^[1] ; Fabienne,Archer ^[2] ; Adélie,Colin ^[3] ; Alexandra,Bobet-Erny ^[2] ; Angélique,Champavère ^[2] ; Edwige,Gros ^[1] ; Pascale,Beurdeley ^[1] ; Stéphane,Cruveiller ^[1] ; Florence,Tardy ^[3] ; Marc,Eloit ^[4]

作者单位： PathoQuest, Paris, France. ^[1] University of Lyon, Université Claude Bernard Lyon1, INRAE, EPHE, IVPC, Viral Infections and Comparative Pathology, UMR754, F69007, Lyon, France. ^[2] Université de Lyon, Anses Laboratoire de Lyon, VetAgro Sup, UMR Mycoplasmoses des Ruminants, F69364, Lyon, France. ^[3] PathoQuest, Paris, France; Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, 94704 Cedex, France; Pathogen Discovery Laboratory, Institut Pasteur, Paris, France. Electronic address: marc.eloit@pathoquest.com. ^[4]

关键词 4-Thiouridine Biologicals High throughput sequencing Metagenomics Mycoplasma RNA-Seq Sterility