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A qPCR assay for Bordetella pertussis cells that enumerates both live and dead bacteria.

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第一作者： Stacy,Ramkissoon

第一单位： Milner Centre for Evolution, University of Bath, Bath, United Kingdom.;Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom.

作者： Stacy,Ramkissoon ^[1] ; Iain,MacArthur ^[1] ; Muktar,Ibrahim ^[2] ; Hans,de Graaf ^[2] ; Robert C,Read ^[2] ; Andrew,Preston ^[1]

作者单位： Milner Centre for Evolution, University of Bath, Bath, United Kingdom.;Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom. ^[1] Faculty of Medicine and Institute for Life Sciences, University of Southampton, Southampton, United Kingdom.;School of Clinical Experimental Sciences, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom.;NIHR Clinical Research Facility, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom.;NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust, Southampton General Hospital, University of Southampton, Southampton, United Kingdom. ^[2]

医学主题词百日咳博德特菌(Bordetella pertussis);人类(Humans);分子诊断技术(Molecular Diagnostic Techniques);聚合酶链反应(Polymerase Chain Reaction);敏感性与特异性(Sensitivity and Specificity)

DOI 10.1371/journal.pone.0232334

PMID 32353041

发布时间 2025-05-30

基金项目

MR/N013204/1/MRC_/Medical Research Council/United Kingdom MR/N026993/1/MRC_/Medical Research Council/United Kingdom

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