CRISPR-Cas fluorescent cleavage assay coupled with recombinase polymerase amplification for sensitive and specific detection of <i>Enterocytozoon hepatopenaei</i>.
作者:
关键词
CRISPR, clustered regularly interspaced short palindromic repeatsCRISPR-Cas12aCas, CRISPR-associated proteinEHP, Enterocytozoon hepatopenaeiEca, Enterospora canceriEnterocytozoon hepatopenaeiFB, FAM-ssDNA-Biotin reporterFQ, fluorescent-quencher reporterHer, Hepatospora eriocheirIHHNV, infectious hypodermal and hematopoietic necrosis virusLFD, lateral flow dipstickLateral flow detectionNTC, no-template controlPAM, protospacer adjacent motifRPARPA, recombinase polymerase amplificationRPA-Cas12a, RPA coupled with Cas12a cleavage assaySWP-PCR, nested PCR targeting swpWSSV, white spot syndrome virusptp2, polar tube protein 2swp, spore wall protein
DOI
10.1016/j.btre.2020.e00485
PMID
32577410
发布时间
2024-03-29
- 浏览6
Biotechnology reports (Amsterdam, Netherlands)
2020年27卷
e00485页
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