Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.

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作者： Tessa,Keenan ^[1] ; Fabio,Parmeggiani ^[2] ; Julien,Malassis ^[3] ; Clement Q,Fontenelle ^[3] ; Jean-Baptiste,Vendeville ^[3] ; Wendy,Offen ^[1] ; Peter,Both ^[2] ; Kun,Huang ^[2] ; Andrea,Marchesi ^[2] ; Alex,Heyam ^[1] ; Carl,Young ^[4] ; Simon J,Charnock ^[4] ; Gideon J,Davies ^[1] ; Bruno,Linclau ^[3] ; Sabine L,Flitsch ^[5] ; Martin A,Fascione ^[6]

作者单位： Department of Chemistry, University of York, Heslington, York YO10 5DD, UK. ^[1] Manchester Institute of Biotechnology (MIB), School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. ^[2] School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK. ^[3] Prozomix Limited, Haltwhistle Industrial Estate, Haltwhistle, Northumberland NE49 9HA, UK. ^[4] Manchester Institute of Biotechnology (MIB), School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. Electronic address: sabine.flitsch@manchester.ac.uk. ^[5] Department of Chemistry, University of York, Heslington, York YO10 5DD, UK. Electronic address: martin.fascione@york.ac.uk. ^[6]

关键词 biocatalysis enzyme discovery fluorinated carbohydrates glycobiology kinases oligosaccharides sugar phosphates

主题词生物催化(Biocatalysis);催化域(Catalytic Domain);氟(Fluorine);半乳糖激酶(Galactokinase);卤化作用(Halogenation);动力学(Kinetics);磁共振波谱学(Magnetic Resonance Spectroscopy);单糖类(Monosaccharides);磷酰化(Phosphorylation);磷酸转移酶类(Phosphotransferases);底物特异性(Substrate Specificity)

DOI 10.1016/j.chembiol.2020.06.005

PMID 32619452

发布时间 2021-07-01

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Cell chemical biology

2020年27卷9期

1199-1206.e5页

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Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.

Cell chemical biology

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Profiling Substrate Promiscuity of Wild-Type Sugar Kinases for Multi-fluorinated Monosaccharides.

Cell chemical biology

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Cell chemical biology

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