The indigoidine synthetase BpsA provides a colorimetric ATP assay that can be adapted to quantify the substrate preferences of other NRPS enzymes.

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作者： Alistair S,Brown ^[1] ; Mark J,Calcott ^[2] ; Vincent M,Collins ^[1] ; Jeremy G,Owen ^[2] ; David F,Ackerley ^[1]

作者单位： School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand. ^[1] Centre for Biodiscovery, Victoria University of Wellington, Wellington, New Zealand. ^[2] School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand. david.ackerley@vuw.ac.nz. ^[3] Centre for Biodiscovery, Victoria University of Wellington, Wellington, New Zealand. david.ackerley@vuw.ac.nz. ^[4]

关键词 ATP Adenylation BpsA Glutamine Indigoidine Microplate assay NRPS Phosphopantetheinyl transferase

主题词腺苷三磷酸(Adenosine Triphosphate);生物传感技术(Biosensing Techniques);比色法(Colorimetry);谷氨酰胺(Glutamine);肽合酶类(Peptide Synthases);哌啶酮类(Piperidones)

DOI 10.1007/s10529-020-02972-4

PMID 32681380

发布时间 2021-07-27

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