Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins.

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第一作者： Kamil,Charubin

第一单位： Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA.;Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.

作者： Kamil,Charubin ^[1] ; Hannah,Streett ^[2] ; Eleftherios Terry,Papoutsakis ^[3]

作者单位： Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA.;Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA. ^[1] Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.;Department of Biological Sciences, University of Delaware, Newark, Delaware, USA. ^[2] Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA epaps@udel.edu.;Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA.;Department of Biological Sciences, University of Delaware, Newark, Delaware, USA. ^[3]

关键词 Clostridium Clostridium acetobutylicum Clostridium ljungdahlii FAST HaloTag SNAP-tag anaerobic fluorescent reporters flow cytometry fluorescent protein super-resolution microscopy