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Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time.

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作者单位: Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. kbarnes@broadinstitute.org. [1] Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA. kbarnes@broadinstitute.org. [2] MRC-University of Glasgow Centre for Virus Research, Glasgow, UK. kbarnes@broadinstitute.org. [3] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. annalach@stanford.edu. [4] Stanford University School of Medicine, Stanford, California, USA. annalach@stanford.edu. [5] Ragon Institute of MGH, MIT, and Harvard, Cambridge, Massachusetts, 02139, USA. [6] Ph.D. Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, Massachusetts, 02115, USA. [7] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [8] Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [9] Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA. [10] Center for Genome Sciences, The United States Army Medical Research Institute for Infectious Disease, 1425 Porter Street, Fort Detrick, Maryland, 21702, USA. [11] Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA. [12] Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA. [13] African Center of Excellence for Genomics of Infectious Disease (ACEGID), Redeemer's University, Ede, Osun State, Nigeria. [14] Department of Biological Sciences, College of Natural Sciences, Redeemer's University, Ede, Osun State, Nigeria. [15] Institute of Lassa Fever Research and Control, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria. [16] Viral Hemorrhagic Fever Program, Kenema Government Hospital, Kenema, Sierra Leone. [17] Department of Electrical Engineering and Computer Science, MIT, Cambridge, Massachusetts, 02139, USA. [18] Zalgen Labs, Germantown, Maryland, USA. [19] Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA. [20] Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA. [21] Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA. [22] Henry M. Jackson Foundation Medical Research International, Abuja, Nigeria. [23] Ministry of Health and Sanitation, Freetown, Sierra Leone. [24] Tulane University School of Medicine, New Orleans, Los Angeles, 70112, USA. [25] Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, Massachusetts, USA. [26] Howard Hughes Medical Institute, Chevy Chase, Maryland, USA. [27]
DOI 10.1038/s41467-020-17994-9
PMID 32807807
发布时间 2021-08-17
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