Efficient genome editing in filamentous fungi via an improved CRISPR-Cas9 ribonucleoprotein method facilitated by chemical reagents.

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作者： Gen,Zou ^[1] ; Meili,Xiao ^[2] ; Shunxing,Chai ^[1] ; Zhihua,Zhu ^[3] ; Ying,Wang ^[1] ; Zhihua,Zhou ^[3]

作者单位： CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Science, Fenglin Rd 300, Shanghai, 200032, China. ^[1] Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agriculture Science, 1000 Jinqi Rd, Fengxian, Shanghai, 201403, China. ^[2] University of Chinese Academy of Sciences, Beijing, 100049, China. ^[3]

主题词冬虫夏草属(Cordyceps);真菌(Fungi);肉座菌目(Hypocreales);原生质体(Protoplasts);核糖核蛋白类(Ribonucleoproteins)

DOI 10.1111/1751-7915.13652

PMID 32841542

发布时间 2024-04-04

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