Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

导出 LinkOut

翻译打印收藏纠错

作者： Xiaowei,Zhang ^[1] ; Ping,Zhang ^[2] ; Lin,An ^[1] ; Ningyuan,Sun ^[2] ; Liying,Peng ^[1] ; Weiwei,Tang ^[2] ; Dingyuan,Ma ^[1] ; Jun,Chen ^[2]

作者单位： State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. ^[1] Department of Pharmacognosy, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, China. ^[2] Department of Prenatal Diagnosis, Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, Nanjing 210004, China. ^[3]

关键词 7-AAD, 7-aminoactinomycin D AKT, AKT serine/threonine kinase, also known as protein kinase B ANOVA, analysis of variance BAX, BCL2-associated X CCK-8, cell counting kit-8 CRISPR, clustered regularly interspaced short palindromic repeats Cas9, caspase 9 Cell death DCFH-DA, dye 2,7-dichloro?uoresce diacetate DMEM, Dulbecco's modified Eagle's medium DMSO, dimethyl sulfoxide ECL, enhanced chemiluminescence ERK1/2, extracellular regulated protein kinases 1/2 FBS, fetal bovine serum FITC, fluorescein isothiocyanate GAPDH, glyceraldehyde-3-phosphate dehydrogenase GSDMD, gasdermin D GSDME GSDME, gasdermin E H&E, hematoxylin and eosin HCC, hepatocellular carcinoma HRP, horseradish peroxidase HepG2 Hepa1-6 Hepatocellular carcinoma IC50, the half maximal inhibitory concentration IgG (H?+?L), immunoglobulin G (heavy chain?+?light chain)KO, knockout LDH, lactic dehydrogenase MEK, mitogen-activated and extracellular signal-regulated kinase MEM, minimum essential medium MMP, mitochondrial membrane potential MS, mass spectrum Miltirone N-GSDME, N-terminal GSDME NAC, N-acetyl cysteine NC, negative control NMR, nuclear magnetic resonance NS, no significance PARP, poly ADP-ribose polymerase PBS, phosphate-based buffer PI, propidium iodide PI3K, phosphatidylinositol 3-kinase Pyroptosis RIPA, radioimmunoprecipitation assay ROS, reactive oxygen species SD, standard deviation SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis TBST, Tris-buffered saline with Tween solution TCGA, the Cancer Genome Atlas VEGF, vascular endothelial growth factor gRNA, guide RNA i.p., intraperitoneal i.v., intravenous mTOR, mammalian target of rapamycin p-AKT, phosphorylated-AKT p-ERK1/2, phosphorylated-ERK1/2 p-MEK, phosphorylated-MEK

DOI 10.1016/j.apsb.2020.06.015

PMID 32963939

发布时间 2020-09-28

翻译满意度评价：

准确一般字义偏差语意偏离译文作用小提交

浏览10

Acta pharmaceutica Sinica. B

2020年10卷8期

1397-1413页

相似文献

中文期刊
外文期刊
学位论文
会议论文

检索历史清除

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

Acta pharmaceutica Sinica. B

相似文献

Acta pharmaceutica Sinica. B

相关期刊

参考文献：
指定格式：	NoteExpress EndNote RefWorks NoteFirst

检索历史 清除

Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis.

Acta pharmaceutica Sinica. B

相似文献

Acta pharmaceutica Sinica. B

相关期刊

检索历史清除