A new hybrid immunocapture bioassay with improved reproducibility to measure tissue factor-dependent procoagulant activity of microvesicles from body fluids.

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作者： Corentin,Franco ^[1] ; Romaric,Lacroix ^[2] ; Loris,Vallier ^[3] ; Coralie,Judicone ^[4] ; Tarik,Bouriche ^[5] ; Sophie,Laroumagne ^[6] ; Philippe,Astoul ^[7] ; Francoise,Dignat-George ^[8] ; Philippe,Poncelet ^[9]

作者单位： Aix Marseille Univ, INSERM, INRAE, C2VN, Marseille, France; BioCytex, Research and Technology Department, Marseille, France. Electronic address: Corentin.franco@biocytex.fr. ^[1] Aix Marseille Univ, INSERM, INRAE, C2VN, Marseille, France; Aix Marseille Univ, APHM, CHU La Conception, Department of Hematology and Vascular Biology, Marseille, France. Electronic address: Romaric.lacroix@univ-amu.fr. ^[2] Aix Marseille Univ, INSERM, INRAE, C2VN, Marseille, France; Aix Marseille Univ, APHM, CHU La Conception, Department of Hematology and Vascular Biology, Marseille, France. ^[3] BioCytex, Research and Technology Department, Marseille, France. Electronic address: Coralie.judicone@biocytex.fr. ^[4] BioCytex, Research and Technology Department, Marseille, France. Electronic address: Tarik.bouriche@biocytex.fr. ^[5] Aix Marseille Univ, APHM, Hôpital Nord, Division of Thoracic Oncology, Pleural Diseases, and Interventional Pulmonology, Marseille, France. Electronic address: Sophie.LAROUMAGNE@mail.ap-hm.fr. ^[6] Aix Marseille Univ, APHM, Hôpital Nord, Division of Thoracic Oncology, Pleural Diseases, and Interventional Pulmonology, Marseille, France. Electronic address: Philippe.astoul@ap-hm.fr. ^[7] Aix Marseille Univ, INSERM, INRAE, C2VN, Marseille, France; Aix Marseille Univ, APHM, CHU La Conception, Department of Hematology and Vascular Biology, Marseille, France. Electronic address: francoise.dignat-george@univ-amu.fr. ^[8] BioCytex, Research and Technology Department, Marseille, France. Electronic address: Philippe.poncelet@biocytex.fr. ^[9]

关键词 Extracellular vesicle Functional assay Immunocapture Microvesicle Reproducibility Tissue factor

主题词生物学鉴定法(Biological Assay);细胞源性微粒(Cell-Derived Microparticles);人类(Humans);血浆(Plasma);结果可重复性(Reproducibility of Results);凝血致活酶(Thromboplastin)

DOI 10.1016/j.thromres.2020.09.020

PMID 33038585

发布时间 2021-06-17

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