Counter-Selection Method for Markerless Allelic Exchange in Bordetella bronchiseptica Based on sacB Gene From Bacillus subtilis.

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作者： Nicolás,Ambrosis ^[1] ; Julieta,Fernández ^[1] ; Federico,Sisti ^[1]

作者单位： Instituto de Biotecnología y Biología Molecular (IBBM)-CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. ^[1]

关键词 Bordetella bronchiseptica allelic exchange genetic engineering markerless deletions sucrose counter-selection

主题词枯草芽孢杆菌(Bacillus subtilis);细菌学技术(Bacteriological Techniques);支气管炎博德特菌(Bordetella bronchiseptica);基因敲除技术(Gene Knockout Techniques);基因, 细菌(Genes, Bacterial);遗传工程(Genetic Engineering);己糖基转移酶类(Hexosyltransferases);聚合酶链反应(Polymerase Chain Reaction)

DOI 10.1002/cpmc.125

PMID 33166051

发布时间 2021-07-12

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Current protocols in microbiology

2020年59卷1期

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Counter-Selection Method for Markerless Allelic Exchange in Bordetella bronchiseptica Based on sacB Gene From Bacillus subtilis.

Current protocols in microbiology

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Current protocols in microbiology

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Counter-Selection Method for Markerless Allelic Exchange in Bordetella bronchiseptica Based on sacB Gene From Bacillus subtilis.

Current protocols in microbiology

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Current protocols in microbiology

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