Conformational dynamics during high-fidelity DNA replication and translocation defined using a DNA polymerase with a fluorescent artificial amino acid.

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作者： Tyler L,Dangerfield ^[1] ; Kenneth A,Johnson ^[2]

作者单位： Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, Austin, Texas, USA. ^[1] Institute for Cellular and Molecular Biology, Department of Molecular Biosciences, University of Texas, Austin, Texas, USA. Electronic address: kajohnson@mail.utexas.edu. ^[2]

关键词 DNA polymerase DNA replication bacteriophage T7 conformational change fluorescent unnatural amino acid pre-steady-state kinetics, global data fitting, free energy profile.pyrophosphorolysis translocation

主题词氨基酸类(Amino Acids);细菌噬菌体T7(Bacteriophage T7);DNA复制(DNA Replication);指导DNA的DNA聚合酶(DNA-Directed DNA Polymerase);荧光(Fluorescence);荧光染料(Fluorescent Dyes);蛋白质构象(Protein Conformation);底物特异性(Substrate Specificity);热力学(Thermodynamics)

DOI 10.1074/jbc.RA120.016617

PMID 33273013

发布时间 2021-08-20

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The Journal of biological chemistry

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Conformational dynamics during high-fidelity DNA replication and translocation defined using a DNA polymerase with a fluorescent artificial amino acid.

The Journal of biological chemistry

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The Journal of biological chemistry

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Conformational dynamics during high-fidelity DNA replication and translocation defined using a DNA polymerase with a fluorescent artificial amino acid.

The Journal of biological chemistry

相似文献

The Journal of biological chemistry

相关期刊

检索历史清除