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Single probe PCR melting curve analysis MTHFR C677T SNP sites.

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第一作者： Kai-Yun,Chen

第一单位： Department of Drug Clinical Trial, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.

作者： Kai-Yun,Chen ^[1] ; Jiang-Xia,Xu ^[2] ; Meng-Meng,Wang ^[3] ; Die,Hu ^[4] ; Fangfang,Xie ^[4] ; Defa,Huang ^[4] ; Jie,Chen ^[4] ; Tong,Yang ^[4] ; Jiajian,Zhang ^[5] ; Fangli,Song ^[5] ; Shao,Huang ^[5] ; Tianyu,Zhong ^[6]

作者单位： Department of Drug Clinical Trial, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China. ^[1] Department of Laboratory Medicine, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China. ^[2] Department of Pathology, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China. ^[3] The First School of Clinical Medicine, Gannan Medical University, Ganzhou, Jiangxi Province, China. ^[4] Jiangxi Shiningmed Com Ltd, China. ^[5] Department of Laboratory Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi Province, China. Electronic address: zhongtianyu@126.com. ^[6]

关键词 Direct blood PCR Folic acid MTHFR C677T Melting curve TaqMan-probe

医学主题词女(雌)性(Female);人类(Humans);男(雄)性(Male);亚甲基四氢叶酸还原酶(NADPH)(Methylenetetrahydrofolate Reductase (NADPH2));核酸变性作用(Nucleic Acid Denaturation);聚合酶链反应(Polymerase Chain Reaction);多态性, 单核苷酸(Polymorphism, Single Nucleotide)

DOI 10.1016/j.ab.2021.114102

PMID 33450284

发布时间 2021-07-08

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