No detectable alloreactive transcriptional responses under standard sample preparation conditions during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells.

导出 OA

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作者： Christopher S,McGinnis ^[1] ; David A,Siegel ^[2] ; Guorui,Xie ^[3] ; George,Hartoularos ^[4] ; Mars,Stone ^[5] ; Chun J,Ye ^[6] ; Zev J,Gartner ^[7] ; Nadia R,Roan ^[8] ; Sulggi A,Lee ^[5]

作者单位： Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA, USA. ^[1] Department of Medicine, Division of HIV/AIDS, UCSF, San Francisco, CA, USA. ^[2] Gladstone Institute of Virology, San Francisco, CA, USA. ^[3] Department of Urology, UCSF, San Francisco, CA, USA. ^[4] Institute for Human Genetics, UCSF, San Francisco, CA, USA. ^[5] Graduate Program in Biological and Medical Informatics, UCSF, San Francisco, CA, USA. ^[6] Department of Laboratory Medicine, UCSF, San Francisco, CA, USA. ^[7] Vitalant Research Institute, UCSF, San Francisco, CA, USA. ^[8] Department of Epidemiology and Biostatistics, UCSF, San Francisco, CA, USA. ^[9] Department of Bioengineering and Therapeutic Sciences, UCSF, San Francisco, CA, USA. ^[10] Parker Institute for Cancer Immunotherapy, San Francisco, CA, USA. ^[11] Chan Zuckerberg BioHub, UCSF, San Francisco, CA, USA. ^[12] Center for Cellular Construction, UCSF, San Francisco, CA, USA. ^[13] Helen Diller Family Comprehensive Cancer Center, San Francisco, CA, USA. ^[14] Gladstone Institute of Virology, San Francisco, CA, USA. nadia.roan@ucsf.edu. ^[15] Department of Urology, UCSF, San Francisco, CA, USA. nadia.roan@ucsf.edu. ^[16] Department of Medicine, Division of HIV/AIDS, UCSF, San Francisco, CA, USA. sulggi.lee@ucsf.edu. ^[17]

关键词 Alloreactivity PBMCs Sample multiplexing Sample preparation scRNA-seq

主题词人类(Humans);白细胞, 单核(Leukocytes, Mononuclear);序列分析, RNA(Sequence Analysis, RNA);单细胞分析(Single-Cell Analysis);标本制备(Specimen Handling)

DOI 10.1186/s12915-020-00941-x

PMID 33472616

发布时间 2024-05-07

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