A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase.

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作者： András Dávid,Tóth ^[1] ; Dániel,Garger ^[2] ; Susanne,Prokop ^[3] ; Eszter,Soltész-Katona ^[4] ; Péter,Várnai ^[4] ; András,Balla ^[4] ; Gábor,Turu ^[4] ; László,Hunyady ^[5]

作者单位： Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; MTA-SE Laboratory of Molecular Physiology, Eötvös Loránd Research Network, Budapest, Hungary; Department of Internal Medicine and Hematology, Semmelweis University, Budapest, Hungary. ^[1] Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary. ^[2] Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; Szentágothai János Doctoral School of Neuroscience, Semmelweis University, Budapest, Hungary. ^[3] Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; MTA-SE Laboratory of Molecular Physiology, Eötvös Loránd Research Network, Budapest, Hungary. ^[4] Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary; MTA-SE Laboratory of Molecular Physiology, Eötvös Loránd Research Network, Budapest, Hungary. Electronic address: hunyady.laszlo@med.semmelweis-univ.hu. ^[5]

关键词 G protein–coupled receptor (GPCR)Gaussia luciferase (GLuc)NanoLuciferase (NanoLuc)bioluminescence resonance energy transfer (BRET)cell surface receptor ligand binding molecular pharmacology signal transduction

主题词生物学鉴定法(Biological Assay);细胞膜(Cell Membrane);能量传递(Energy Transfer);HEK293细胞(HEK293 Cells);人类(Humans);动力学(Kinetics);配体(Ligands);萤光素酶类(Luciferases);蛋白质结合(Protein Binding);蛋白质转运(Protein Transport);受体, G-蛋白偶联(Receptors, G-Protein-Coupled);信号传导(Signal Transduction)

DOI 10.1016/j.jbc.2021.100366

PMID 33545176

发布时间 2024-04-01

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The Journal of biological chemistry

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A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase.

The Journal of biological chemistry

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A general method for quantifying ligand binding to unmodified receptors using Gaussia luciferase.

The Journal of biological chemistry

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The Journal of biological chemistry

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