Verification of CRISPR editing and finding transgenic inserts by Xdrop indirect sequence capture followed by short- and long-read sequencing.

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第一作者： Thorarinn,Blondal

第一单位： Samplix ApS, Mileparken 28, Herlev, Denmark.

作者： Thorarinn,Blondal ^[1] ; Cristina,Gamba ^[1] ; Lea,Møller Jagd ^[1] ; Ling,Su ^[2] ; Dimiter,Demirov ^[2] ; Shuang,Guo ^[2] ; Camille M,Johnston ^[1] ; Eva M,Riising ^[1] ; Xiaolin,Wu ^[2] ; Marie J,Mikkelsen ^[1] ; Ludmila,Szabova ^[3] ; Peter,Mouritzen ^[4]

作者单位： Samplix ApS, Mileparken 28, Herlev, Denmark. ^[1] Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA. ^[2] Center for Advanced Preclinical Research, Frederick National Laboratory for Cancer Research at the National Cancer Institute-Frederick, Frederick, MD, USA. ^[3] Samplix ApS, Mileparken 28, Herlev, Denmark. Electronic address: pmo@samplix.com. ^[4]

关键词 CRISPR editing validation Indirect sequence capture Long-read sequencing Loss of heterozygosity Target enrichment

主题词动物(Animals);动物, 基因修饰(Animals, Genetically Modified);小鼠(Mice)

DOI 10.1016/j.ymeth.2021.02.003

PMID 33582298

发布时间 2022-01-04

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