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Construction and comprehensive characterization of an EcLDCc-CatIB set-varying linkers and aggregation inducing tags.

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第一作者： Kira,Küsters

第一单位： Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.;Institute of Biotechnology, RWTH Aachen University, 52074, Aachen, Germany.

作者： Kira,Küsters ^[1] ; Martina,Pohl ^[2] ; Ulrich,Krauss ^[3] ; Gizem,Ölçücü ^[3] ; Sandor,Albert ^[4] ; Karl-Erich,Jaeger ^[3] ; Wolfgang,Wiechert ^[5] ; Marco,Oldiges ^[6]

作者单位： Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.;Institute of Biotechnology, RWTH Aachen University, 52074, Aachen, Germany. ^[1] Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany. ^[2] Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.;Institute of Molecular Enzyme Technology, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany. ^[3] Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.;Faculty of Biotechnology, University of Applied Sciences Mannheim, 68163, Mannheim, Germany. ^[4] Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.;Computational Systems Biotechnology (AVT.CSB), RWTH Aachen University, 52074, Aachen, Germany. ^[5] Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany. m.oldiges@fz-juelich.de.;Institute of Biotechnology, RWTH Aachen University, 52074, Aachen, Germany. m.oldiges@fz-juelich.de. ^[6]

关键词 Catalytically active inclusion bodies Downstream processing Enzymes Immobilization Microscopic analysis Protein aggregates Protein engineering

医学主题词羧基裂合酶类(Carboxy-Lyases);大肠杆菌(Escherichia coli);包涵体(Inclusion Bodies)

DOI 10.1186/s12934-021-01539-w

PMID 33596923

发布时间 2021-09-06

基金项目

34.EFRE-0300096/State of North Rhine Westphalia (NRW) and the European Regional Development Fund (EFRE), Project "CLIB-Kompetenzzentrum Biotechnologie (CKB) 34.EFRE-0300097/State of North Rhine Westphalia (NRW) and the European Regional Development Fund (EFRE), Project "CLIB-Kompetenzzentrum Biotechnologie (CKB)

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