Optimization strategies for expression and purification of soluble N-terminal domain of human centriolar protein SAS-6 in Escherichia coli.

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作者： Eswar Reddy,Maddi ^[1] ; Ramanathan,Natesh ^[2]

作者单位： School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Thiruvananthapuram, 695551, Kerala, India. ^[1] School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Thiruvananthapuram, 695551, Kerala, India. Electronic address: natesh@iisertvm.ac.in. ^[2]

关键词 Affinity tag Fixed arm approach Fusion protein Improved solubility MBP N-terminal SAS-6 SUMO tag Solubility-enhancing tag Surface entropy reduction

主题词细胞周期蛋白质类(Cell Cycle Proteins);大肠杆菌(Escherichia coli);基因表达(Gene Expression);人类(Humans);重组蛋白质类(Recombinant Proteins);溶解度(Solubility)

DOI 10.1016/j.pep.2021.105856

PMID 33640460

发布时间 2021-12-24

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