Expression and purification of soluble and active human enterokinase light chain in <i>Escherichia coli</i>.

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作者： Young Su,Kim ^[1] ; Hye-Jeong,Lee ^[2] ; Sang-Hyun,Park ^[2] ; Yeu-Chun,Kim ^[2] ; Jungoh,Ahn ^[3]

作者单位： Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea. ^[1] Biotechnology Process Engineering Center, KRIBB, Cheongju 28116, Republic of Korea. ^[2] Department of Bioprocess Engineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. ^[3]

关键词 D4K, (Aspartic Acid)4 Lysine EK, enterokinase Escherichia coli Fusion technology Human enterokinase light chain IPTG, isopropyl β-D-1-thiogalactopyranoside MBP, maltose-binding protein Recombinant protein Self-cleavage TEV, tobacco etch virus bEKL, bovine enterokinase light chain hEKL, human enterokinase light chain

DOI 10.1016/j.btre.2021.e00626

PMID 34026576

发布时间 2021-05-26

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Biotechnology reports (Amsterdam, Netherlands)

2021年30卷

e00626页

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Expression and purification of soluble and active human enterokinase light chain in <i>Escherichia coli</i>.

Biotechnology reports (Amsterdam, Netherlands)

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Biotechnology reports (Amsterdam, Netherlands)

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Expression and purification of soluble and active human enterokinase light chain in <i>Escherichia coli</i>.

Biotechnology reports (Amsterdam, Netherlands)

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Biotechnology reports (Amsterdam, Netherlands)

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