A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping.

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作者： Clark H,Cunningham ^[1] ; Christopher M,Hennelly ^[1] ; Jessica T,Lin ^[1] ; Ratawan,Ubalee ^[2] ; Ross M,Boyce ^[3] ; Edgar M,Mulogo ^[4] ; Nicholas,Hathaway ^[5] ; Kyaw L,Thwai ^[1] ; Fernandine,Phanzu ^[6] ; Albert,Kalonji ^[6] ; Kashamuka,Mwandagalirwa ^[7] ; Antoinette,Tshefu ^[7] ; Jonathan J,Juliano ^[1] ; Jonathan B,Parr ^[8]

作者单位： University of North Carolina at Chapel Hill, Chapel Hill, NC, United States. ^[1] Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. ^[2] University of North Carolina at Chapel Hill, Chapel Hill, NC, United States; Mbarara University of Science and Technology, Mbarara, Uganda. ^[3] Mbarara University of Science and Technology, Mbarara, Uganda. ^[4] University of Massachusetts School of Medicine, Worcester, MA, United States. ^[5] SANRU ASBL (Global Fund), Kinshasa, Democratic Republic of the Congo. ^[6] Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo. ^[7] University of North Carolina at Chapel Hill, Chapel Hill, NC, United States. Electronic address: jonathan_parr@med.unc.edu. ^[8]

关键词 CRISPR Cas13a Diagnostic Malaria SHERLOCK

主题词碱基配对(Base Pairing);刚果(Congo);DNA, 原虫(DNA, Protozoan);刚果民主共和国(Democratic Republic of the Congo);诊断试验, 常规(Diagnostic Tests, Routine);二氢蝶酸合酶(Dihydropteroate Synthase);抗药性(Drug Resistance);早期诊断(Early Diagnosis);人类(Humans);疟疾(Malaria);疟原虫属(Plasmodium);多态性, 单核苷酸(Polymorphism, Single Nucleotide);人群监测(Population Surveillance);敏感性与特异性(Sensitivity and Specificity);种特异性(Species Specificity);磺胺多辛(Sulfadoxine);泰国(Thailand);乌干达(Uganda)

DOI 10.1016/j.ebiom.2021.103415

PMID 34139428

发布时间 2024-08-11

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A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping.

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A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, species differentiation, and drug-resistance genotyping.

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