Development and evaluation of a nanopore 16S rRNA gene sequencing service for same day targeted treatment of bacterial respiratory infection in the intensive care unit.

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作者： Rossella,Baldan ^[1] ; Penelope R,Cliff ^[2] ; Sarah,Burns ^[1] ; Adela,Medina ^[2] ; Graeme C,Smith ^[2] ; Rahul,Batra ^[1] ; Alberto,Cerda ^[2] ; Rebekah,Wilson ^[2] ; Tammy,Merrill ^[2] ; Shona J,Lewis ^[2] ; Amita,Patel ^[1] ; Dakshika,Jeyaratnam ^[3] ; Duncan L,Wyncoll ^[4] ; Nicholas,Barrett ^[4] ; Meera A,Chand ^[5] ; Jonathan D,Edgeworth ^[6]

作者单位： Centre for Clinical Infection and Diagnostics Research, Department of Infectious Diseases, King's College London, St Thomas' Hospital, Westminster Bridge Road, London, UK; Department of Infectious Diseases, Guy's and St Thomas' NHS Foundation Trust, London, UK. ^[1] Infection Sciences, Viapath, St Thomas' Hospital, London, UK. ^[2] South London Specialist Virology Centre, King's College Hospital NHS Foundation Trust, London, UK. ^[3] Department of Critical Care, Guy's and St Thomas' NHS Foundation Trust, London, UK. ^[4] Department of Infectious Diseases, Guy's and St Thomas' NHS Foundation Trust, London, UK; Department of Critical Care, Guy's and St Thomas' NHS Foundation Trust, London, UK; National Infection Service, Public Health England, London, UK; Health Protection Research Unit in Respiratory Infections, Imperial College London, London, UK. ^[5] Centre for Clinical Infection and Diagnostics Research, Department of Infectious Diseases, King's College London, St Thomas' Hospital, Westminster Bridge Road, London, UK; Department of Infectious Diseases, Guy's and St Thomas' NHS Foundation Trust, London, UK; Infection Sciences, Viapath, St Thomas' Hospital, London, UK. Electronic address: Jonathan.Edgeworth@gstt.nhs.uk. ^[6]

关键词 16S rRNA Bacterial infections LRTI Nanopore flongle Rapid diagnostics

主题词基因, rRNA(Genes, rRNA);人类(Humans);重症监护病房(Intensive Care Units);纳米孔(Nanopores);RNA, 核糖体, 16S(RNA, Ribosomal, 16S);结果可重复性(Reproducibility of Results)

DOI 10.1016/j.jinf.2021.06.014

PMID 34146598

发布时间 2021-08-05

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