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Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS.

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第一作者： Sara,Alameldin

第一单位： Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.;Clinical Pathology Department, Assiut University Hospital, Assiut, Egypt.

作者： Sara,Alameldin ^[1] ; Victor,Costina ^[2] ; Hesham A,Abdel-Baset ^[3] ; Katja,Nitschke ^[4] ; Phillip,Nuhn ^[4] ; Michael,Neumaier ^[2] ; Maren,Hedtke ^[2]

作者单位： Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany.;Clinical Pathology Department, Assiut University Hospital, Assiut, Egypt. ^[1] Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany. ^[2] Clinical Pathology Department, Assiut University Hospital, Assiut, Egypt. ^[3] Department of Urology and Urosurgery, Medical Faculty Mannheim of the University of Heidelberg, University Hospital Mannheim, Mannheim, Germany. ^[4]

关键词 AGO2, Argonaute protein CSF, cerebrospinal fluid EVs, extracellular vesicles Exosomes FDR, false discovery rate Human plasma ILVs, intraluminal vesicles MVBs, multivesicular bodies Mass spectrometry SEC, size exclusion chromatography Size-exclusion chromatography UC, ultracentrifugation Ultracentrifugation cfNAs, Cell-free nucleic acids ctDNA, circulating tumor DNA miRNA, microRNA

DOI 10.1016/j.plabm.2021.e00241

PMID 34258353

发布时间 2022-04-24

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Practical laboratory medicine

2021年26卷

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Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS.

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Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS.

Practical laboratory medicine

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Practical laboratory medicine

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