Generation of phytase chimeras with low sequence identities and improved thermal stability.

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作者： Kevin R,Herrmann ^[1] ; Isabell,Hofmann ^[1] ; Dennis,Jungherz ^[1] ; Malte,Wittwer ^[1] ; Belén,Infanzón ^[1] ; Stefanie Nicole,Hamer ^[1] ; Mehdi D,Davari ^[1] ; Anna Joëlle,Ruff ^[2] ; Ulrich,Schwaneberg ^[3]

作者单位： Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany. ^[1] Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany. Electronic address: aj.ruff@biotec.rwth-aachen.de. ^[2] Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany; DWI-Leibniz Institut für Interaktive Materialien, Forckenbeckstraße 50, 52056, Aachen, Germany. Electronic address: u.schwaneberg@biotec.rwth-aachen.de. ^[3]

关键词 Chimera DNA recombination PTRec Phytase Sequence identity Thermal stability

主题词 6-植酸酶(6-Phytase);柠檬酸杆菌属(Citrobacter);酶稳定性(Enzyme Stability);蜂房哈夫尼亚菌(Hafnia alvei);氢离子浓度(Hydrogen-Ion Concentration);重组融合蛋白质类(Recombinant Fusion Proteins);耶尔森菌属(Yersinia)

DOI 10.1016/j.jbiotec.2021.07.005

PMID 34271055

发布时间 2022-05-31

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