Tailored nanodisc immobilization for one-step purification and reconstitution of cytochrome P450: A tool for membrane proteins' hard cases.

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第一作者： Lan,Zhao

第一单位： State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China.

作者： Lan,Zhao ^[1] ; Jiaoli,Tao ^[2] ; Yongdong,Huang ^[1] ; Kai,Zhu ^[1] ; Yuxiang,Du ^[2] ; Dongxia,Hao ^[1] ; Hongying,Liu ^[3] ; Rongyue,Zhang ^[4] ; Guanghui,Ma ^[5]

作者单位： State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China. ^[1] State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China.;School of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing, P. R. China. ^[2] School of Chemical and Environmental Engineering, China University of Mining and Technology, Beijing, P. R. China. ^[3] Department of Applied Chemistry, Beijing Institute of Petrochemical Technology, Beijing, P. R. China. ^[4] State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, P. R. China.;School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing, P. R. China. ^[5]

关键词 cytochrome P450 membrane protein purification nanodisc-immobilized chromatography nanodiscs reconstitution