Ultrarapid detection of SARS-CoV-2 RNA using a reverse transcription-free exponential amplification reaction, RTF-EXPAR.

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第一作者： Jake G,Carter

第一单位： School of Chemistry, University of Birmingham, Birmingham B15 2TT, United Kingdom.

作者： Jake G,Carter ^[1] ; Lorea,Orueta Iturbe ^[2] ; Jean-Louis H A,Duprey ^[3] ; Ian R,Carter ^[3] ; Craig D,Southern ^[4] ; Marium,Rana ^[4] ; Celina M,Whalley ^[1] ; Andrew,Bosworth ^[2] ; Andrew D,Beggs ^[5] ; Matthew R,Hicks ^[5] ; James H R,Tucker ^[6] ; Timothy R,Dafforn ^[5]

作者单位： School of Chemistry, University of Birmingham, Birmingham B15 2TT, United Kingdom. ^[1] School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom. ^[2] Linear Diagnostics Ltd, Birmingham B15 2SQ, United Kingdom. ^[3] C2JF Solutions LLP, Liverpool L24 9LG, United Kingdom. ^[4] Institute of Cancer and Genomics, University of Birmingham, Birmingham B15 2TT, United Kingdom. ^[5] Clinical Virology, Clinical Laboratory Services, University Hospitals Birmingham NHS Foundation Trust, Birmingham B15 2GW, United Kingdom. ^[6] School of Chemistry, University of Birmingham, Birmingham B15 2TT, United Kingdom; j.tucker@bham.ac.uk t.r.dafforn@bham.ac.uk. ^[7] School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom; j.tucker@bham.ac.uk t.r.dafforn@bham.ac.uk. ^[8]

关键词 COVID-19 assay EXPAR RNA detection isothermal amplification nucleic acids

主题词人类(Humans);核酸扩增技术(Nucleic Acid Amplification Techniques);RNA, 病毒(RNA, Viral);逆转录作用(Reverse Transcription);敏感性与特异性(Sensitivity and Specificity)

DOI 10.1073/pnas.2100347118

PMID 34400545

发布时间 2021-10-28

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